Citation: Bonini, M., Šikoparija, B., Prentović, M., Cislaghi, G., Colombo, P., Testoni, C., Grewling, Ł., Lommen, S. T. E., Müller-Schärer, H. and Smith, M. (2015). A follow-up study examining airborne Ambrosia pollen in the Milan area in 2014 in relation to the accidental introduction of the ragweed leaf beetle Ophraella communa. Aerobiologia, DOI:10.1007/s10453-015-9406-2
Citation: Zhao F, Elkelish A, Durner J, Lindermayr C, Winkler JB, Lang H, Ruёff F, Behrendt H, Traidl-Hoffmann C, Holzinger A, Kofler W, Braun P, von Törne C, Hauck SM, Ernst D, Frank U (2015) Common ragweed (Ambrosia artemisiifolia L.): Allergenicity and molecular characterization of the pollen after exposure the plants to elevated NO2. Plant Cell & Environment,
Ragweed pollen is the main cause of allergenic diseases in Northern America, and the weed has become a spreading neophyte in Europe. Climate change and air pollution are speculated to affect the allergenic potential of pollen. The objective of this study was to investigate the effects of NO2, a major air pollutant, under controlled conditions, on the allergenicity of ragweed pollen.
Ragweed was exposed to different levels of NO2 throughout the entire growing season, and its pollen further analysed. Spectroscopic analysis showed increased outer cell wall polymers and decreased amounts of pectin. Proteome studies using two-dimensional difference gel electrophoresis and liquid chromatography–tandem mass spectrometry indicated increased amounts of several Amb a 1 isoforms and of another allergen with great homology to enolase Hev b 9 from rubber tree. Analysis of protein S-nitrosylation identified nitrosylated proteins in pollen from both conditions, including Amb a 1 isoforms. However, elevated NO2 significantly enhanced the overall nitrosylation. Finally, we demonstrated increased overall pollen allergenicity by immunoblotting using ragweed antisera, showing a significantly higher allergenicity for Amb a 1. The data highlight a direct influence of elevated NO2 on the increased allergenicity of ragweed pollen and a direct correlation with an increased risk for human health.
Citation: Wimmer A, Alessandrini F, Gilles S, Frank U, Oeder S, Hauser M, Ring J, Ferreira F, Ernst D, Winkler JB, Schmitt-Kopplin P, Ohnmacht C, Behrendt H, Schmidt-Weber C, Traidl-Hoffmann C, Gutermuth J (2015) Pollen-derived adenosine is a necessary co-factor for ragweed allergy. Allergy 70, 944-954
Ragweed (Ambrosia artemisiifolia) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence. Various components of ragweed pollen are thought to play a role in the development of allergic responses. The aim of this study was to identify critical factors for allergenicity of ragweed pollen in a physiological model of allergic airway inflammation.
Aqueous ragweed pollen extract, the low molecular weight fraction or the major allergen Amb a 1 was instilled intranasally on 1–11 consecutive days, and allergic airway inflammation was evaluated by bronchoalveolar lavage, lung histology, serology, gene expression in lung tissue, and measurement of lung function. Pollen-derived adenosine was removed from the extract enzymatically to analyze its role in ragweed-induced allergy. Migration of human neutrophils and eosinophils toward supernatants of ragweed-stimulated bronchial epithelial cells was analyzed.
Instillation of ragweed pollen extract, but not of the major allergen or the low molecular weight fraction, induced specific IgG1, pulmonary infiltration with inflammatory cells, a Th2-associated cytokine signature in pulmonary tissue, and impaired lung function. Adenosine aggravated ragweed-induced allergic lung inflammation. In vitro, human neutrophils and eosinophils migrated toward supernatants of bronchial epithelial cells stimulated with ragweed extract only if adenosine was present.
Pollen-derived adenosine is a critical factor in ragweed-pollen-induced allergic airway inflammation. Future studies aim at therapeutic strategies to control these allergen-independent pathways.
The first National Air Quality Day “La première Journée nationale de la qualité de l’air (JNQA)” in France was held on September 25, 2015. It was one of the actions of the roadmap of the environmental conference that was held in 2014. The purpose of which was to involve citizens in air quality.
To mark the First National Air Quality Day, the RNSA (French Aerobiology Network) held an information stand on pollen and ragweed at Croix Rousse in Lyon. A hundred people visited the stand and many documents about allergies, pollen, air quality and ragweed were distributed. It was a good opportunity to communicate with the general public about biological particles present in the air, and to disseminate information about the health impact of aeroallergens.
From the 16th to 22nd of August 2015 the 8th Advanced Aerobiology Course “From phenology to sophisticated forecasting“ was held in Siauliai University (Lithuania). Seventeen participants (including 4 who received SMARTER grants) from 11 European countries broadened their knowledge and skills in aerobiology. Issues about invasive plants were included in the course.
Leading scientists from the field of aerobiology provided state-of-the-art training sessions in auditoriums. There was also the opportunity to conduct research on invasive species in the field. On the “Botany Day”, fieldwork was performed in the “Kurtuvenai” Natural Park. Course participants investigated forest and meadows but ragweed was not found. Ambrosia modelling was conducted on data from various sources. Information collected during the fieldwork was used in the ecosystem analysis on “Vegetation Day”.
The course ended with the “Data Handling Day” that included reports of participant’s respective pollen information networks or their aerobiological research, and an exam of 20 questions. All participants passed the exam successfully and received their certificates.